Anti-CD3 antibody [CD3-12] ab11089 from Abcam

Product Specs
ItemAnti-CD3 antibody [CD3-12]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab11089
Quantity100 UG
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
FormatPurified IgG prepared from tissue culture supernatant
IsotypeIgG1
CloneCD3-12
HostRat
Antibody TypePrimary
ClonalityMonoclonal
Concentration1 mg/ml
Research AreaImmuno-oncology
Gene NameCD3E
ReactivityHuman, Mouse, Rat
TargetCD3

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Imaging and AI based chromatin biomarkers for diagnosis and therapy evaluation from liquid biopsiesNPJ Precis OncolDecember 14, 2023Kiran Challa, Daniel Paysan, Dominic Leiser, Nadia Sauder, Damien C. Weber, G. V. Shivashankarmouse anti-Lamin A/C from Abcam (catalog no. ab8980), monoclonal rat anti-CD3 from Abcam (catalog no. ab11089), recombinant Alexa Fluor 647 anti-CD8 alpha antibody from Abcam (catalog no. ab196193), monoclonal

(2) Topical Calcipotriol Plus Imiquimod Immunotherapy for Nonkeratinocyte Skin CancersJID InnovationsNovember 1, 2023Marjan Azin, Kenneth H. Ngo, Jennet Hojanazarova, Shadmehr Demehri1 hour and stained with anti-mouse primary antibodies, including anti-CD3 antibody (catalog number ab11089, Abcam, Cambridge, United Kingdom) and anti-CD4 antibody (catalog number ab183685, Abcam) overnight

(3) Novel miRNA-based drug CD5-2 reduces liver tumor growth in diethylnitrosamine-treated mice by normalizing tumor vasculature and altering immune infiltrateFrontiers in ImmunologySeptember 18, 2023Ken Liu, Jinbiao Chen, Yang Zhao, Jade Boland, Ka Ka Ting, Glen Lockwood, Catriona McKenzie, James Kench, Mathew A. Vadas, Jennifer R. Gamble, et al.purchased from Thermo Fisher Scientific (Scoresby, VIC 3179, Australia). Antibodies against CD3 (cat# ab11089), CD34 (cat# ab81289) and carbonic anhydrase 9 (CAIX, cat# ab184006) were bought from Abcam (Cambridge,

(4) The MIF promoter SNP rs755622 is associated with immune activation in glioblastomaJCI insightJuly 10, 2023Alban TJ, Grabowski MM, Otvos B, Bayik D, Wang W, Zalavadia A, Makarov V, Troike K, McGraw M, Rabljenovic A, et al.different sets of markers and indicated below: Set 1 (triple immunofluorescence staining): DAPI, CD3 (ab11089, Abcam, 1:50), CD8 (85336, Cell Signaling, 1:50), CD107a (NBP2-52721, Novus Bio, 1:50); Set 2 (double

(5) Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid glandJCI insightJune 22, 2023Zhang X, Young C, Liao XH, Refetoff S, Torres M, Tomer Y, Stefan-Lifshitz M, Zhang H, Larkin D, Fang D, et al.(ab109510, Abcam); rabbit anti-CD45 (ab10558, Abcam); rat anti-Mac2 (14-5301-81, Invitrogen); rat anti-CD3 (ab11089, Abcam); rat anti-CD8a (14-0808-82, Invitrogen); rat anti-CD19 (14-0194-82, Invitrogen); rabbit anti-P62

(6) Metronomic capecitabine inhibits liver transplant rejection in rats by triggering recipients’ T cell ferroptosisWorld Journal of GastroenterologyMay 28, 2023Hao Wang, Zheng-Lu Wang, Sai Zhang, De-Jun Kong, Rui-Ning Yang, Lei Cao, Jian-Xi Wang, Sei Yoshida, Zhuo-Lun Song, Tao Liu, et al.serum. Then the sections were incubated overnight at 4 ℃ with a rat anti-CD3 antibody (CD3-12, Cat# ab11089, RRID: AB_2889189; Abcam, Cambridge, MA, United States) and incubated with a secondary antibody (Goat Anti-Rat IgG H&L

(7) EphB4 monomer inhibits chronic graft vasculopathy in an aortic transplant modelJVS-Vascular ScienceMay 22, 2023John T. Langford, Luis Gonzalez, Ryosuke Taniguchi, Anand Brahmandam, Weichang Zhang, Alan Dardikhour at room temperature and then incubated overnight at 4°C with either CD68 (ab31630; Abcam) or CD3 (ab11089). Sections were then treated with secondary antibodies at room temperature for 1 hour using Alexa Fluor

(8) Rapid generation of a mouse model for evaluating on-target normal tissue toxicity of human CAR-T cells using replication-defective recombinant adenovirusJournal of advanced researchMay 1, 2023Liao Q, Liu Z, Zhu C, He H, Feng M, Jiang L, Ding X, Sun R, Zhang X, Xu J.CD47 (ab218810, Abcam) for immunohistochemistry (IHC), and immunostained with anti-human CD3 (Cat #: ab11089, Abcam), anti-FLAG tag (Cat #: 637301, Biolegend) antibodies and DAPI for immunofluorescence, which

(9) Selective emergence of antibody-secreting cells in the multiple sclerosis braineBioMedicineMarch 1, 2023Laurens Bogers, Hendrik J. Engelenburg, Malou Janssen, Peter-Paul A. Unger, Marie-José Melief, Annet F. Wierenga-Wolf, Cheng-Chih Hsiao, Matthew R.J. Mason, Jörg Hamann, Jamie van Langelaar, et al.incubated overnight with primary antibody (CD19 (rabbit), 1:200, 11501, Biolegend; CD3 (rat), 1:50, ab11089, Abcam; CXCR5 (mouse), 1:100, MAB190, RD Systems). Endogenous peroxidase activity was quenched in a

(10) CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes ChemoresistanceCancer immunology researchMarch 1, 2023Lao L, Zeng W, Huang P, Chen H, Jia Z, Wang P, Huang D, Chen J, Nie Y, Yang L, et al.(Abcam, #ab17147, 1:100), rabbit-anti-human CD45 (Abcam, #ab10558, 1:100), rat-anti-human CD3 (Abcam, #ab11089, 1:100), rabbit-anti-human CD161 (Abcam, #ab259916, 1:50), mouse-anti-human Vα7.2 (BioLegend, #351702,

(11) Combined PD-1, BRAF and MEK inhibition in BRAF(V600E) colorectal cancer: a phase 2 trialNature medicineFebruary 1, 2023Tian J, Chen JH, Chao SX, Pelka K, Giannakis M, Hess J, Burke K, Jorgji V, Sindurakar P, Braverman J, et al.serum (Sigma-Aldrich) for 1 h at room temperature. The slides were incubated with anti-CD3 (Abcam; ab11089; 1:800 dilution) and anti-CD8 (Cell Signaling; 98941 S; 1:400 dilution) primary antibodies overnight

(12) Infection of SARS-CoV-2 causes severe pathological changes in mouse testisJournal of genetics and genomics = Yi chuan xue baoFebruary 1, 2023Chen M, Li S, Liu S, Zhang Y, Cui X, Lv L, Liu B, Zheng A, Wang Q, Duo S, et al.SOX9 (Millipore, AB5535; Sigma, AMAB90795), Nucleoprotein (Sino biological, 40143-R001), CD3 (Abcam, ab11089), 3β-HSD (Santa Cruz, sc-30820). The secondary antibodies were: FITC-conjugated donkey anti-rabbit IgG

(13) The unforeseen intracellular lifestyle of Enterococcus faecalis in hepatocytesGut MicrobesDecember 31, 2022Natalia Nunez, Aurélie Derré-Bobillot, Nicolas Trainel, Goran Lakisic, Alexandre Lecomte, Françoise Mercier-Nomé, Anne-Marie Cassard, Hélène Bierne, Pascale Serror, Cristel Archambaudmonoclonal anti-claudin 1 (Clinisciences, sc-166338 diluted 1:100), rat monoclonal anti-CD3 (Abcam, ab11089 diluted 1:100) and mouse anti-Ly6G (BioLegend, France, clone A8; diluted 1:50), followed by staining

(14) Cerebrospinal fluid immune dysregulation during healthy brain aging and cognitive impairmentCellDecember 22, 2022Piehl N, van Olst L, Ramakrishnan A, Teregulova V, Simonton B, Zhang Z, Tapp E, Channappa D, Oh H, Losada PM, et al.imaging We stained 5μm paraffin embedded brain tissue sections using antibodies rat anti-CD3 (Abcam ab11089), rabbit anti-CXCR6 (Abcam ab273116), mouse anti-CXCL16 (Thermo MA5–27845), rabbit anti-amyloid-β (Cell Signaling

(15) Sympathetic innervation of human and porcine spleens: implications for between species variation in functionBioelectronic MedicineDecember 19, 2022Logan G. Kirkland, Chloe G. Garbe, Joseph Hadaya, Paul V. Benson, Brant M. Wagener, Sanjin Tankovic, Donald B. Hoover0.4% Triton X-100, 1% BSA, and two primary antibodies (rabbit anti-TH and mouse anti-CD3, 1:20, Abcam ab11089). The ImmPRESS Duet HRP/AP (alkaline phosphatase) Polymer Reagent included a mixture of HRP anti-rabbit

(16) Thymic stromal lymphopoietin-stimulated CD4+ T cells induce senescence in advanced breast cancerFrontiers in Cell and Developmental BiologyNovember 17, 2022Margherita Boieri, Emanuela Marchese, Quan Minh Pham, Marjan Azin, Lauren E. Steidl, Anna Malishkevich, Shadmehr Demehriimmunofluorescence (Guennoun et al., 2022) staining, sections were incubated with CD3 (catalog no. Ab11089, Abcam, Waltham, MA), cytokeratin [catalog no. M3515, Dako (Agilent), Santa Clara, CA, United States]

(17) Cannabidiol markedly alleviates skin and liver fibrosisFrontiers in PharmacologyOctober 19, 2022Carmen del RÃo, Francisco Ruiz-Pino, MarÃa E. Prados, Bernd L. Fiebich, Manuel Tena-Sempere, Eduardo MuÃ±ozwith anti-TNC (1:100) (MAB3138, R&D Systems, Minneapolis, MN, United States), rat anti-CD3 (1:100) (ab11089, Abcam, Cambridge, United Kingdom), or anti-F4/80 (1:50) (MCA497, Bio Rad, Hercules, CA, United States)

(18) STAT3 is Activated by CTGF-mediated Tumor-stroma Cross Talk to Promote HCC ProgressionCellular and Molecular Gastroenterology and HepatologySeptember 20, 2022Yuki Makino, Hayato Hikita, Seiya Kato, Masaya Sugiyama, Minoru Shigekawa, Tatsuya Sakamoto, Yoichi Sasaki, Kazuhiro Murai, Sadatsugu Sakane, Takahiro Kodama, et al.2223021" F4/80,Bio-Rad,"Cat# MCA497RT, RRID:AB 1102558" CD68,Abcam,"Cat# ab955, RRID:AB 307338" CD3,Abcam,"Cat# ab11089, RRID:AB 2889189" PECAM1,Abcam,"Cat# ab28364, RRID:AB 726362" PCNA,Cell Signaling Technology,"Cat#

(19) Ketogenic diet uncovers differential metabolic plasticity of brain cellsScience advancesSeptember 16, 2022Düking T, Spieth L, Berghoff SA, Piepkorn L, Schmidke AM, Mitkovski M, Kannaiyan N, Hosang L, Scholz P, Shaib AH, et al.RRID:AB_887879), Vinculin (Sigma-Aldrich, catalog no. V9131, RRID:AB_477629), CD3 (Abcam, catalog no. ab11089, RRID:AB_2889189), CD3e (BioLegend, catalog no. 100312, clone 145-2C11; RRID:AB_312677), CD4 (Becton Dickinson

(20) Integrated single-cell transcriptomic analyses reveal that GPNMB-high macrophages promote PN-MES transition and impede T cell activation in GBMEBioMedicineSeptember 1, 2022Xiong A, Zhang J, Chen Y, Zhang Y, Yang F.AF2330, RRID:AB_2112934), anti-Mac-3 (1:100, BD, 550292, RRID:AB_393587) and anti-CD3 (1:100, Abcam, ab11089/ab16669, RRID:AB_2889189/AB_443425) overnight at 4 °C. After washing in PBS, sections were stained with

Be the First to Write a Review!

Anti-CD3 antibody [CD3-12] from Abcam

Abcam

Citations (79)

Reviews

Anti-CD3 antibody [CD3-12] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map